Biological Oxygen Demand (BOD)
Biochemical oxygen demand is the amount of dissolved oxygen needed (i.e., demanded) by aerobic biological organisms to break down organic material present in a given water sample at certain temperature over a specific time period.
BOD refers to the amount of oxygen that would be consumed if all the organics in one liter of water were oxidized by bacteria and protozoa.
The first step in measuring BOD is to obtain equal volumes of water from the area to be tested and dilute each specimen with a known volume of distilled water which has been thoroughly shaken to insure oxygen saturation. After this, an oxygen meter is used to determine the concentration of oxygen within one of the vials. The remaining vial is than sealed and placed in darkness and tested five days later. BOD is then determined by subtracting the second meter reading from the first.
The range of possible readings can vary considerably. Water from an exceptionally clear lake might show a BOD of less than 2 ml/L of water. Raw sewage may give readings in the hundreds and food processing wastes may be in the thousands.
The BOD test takes 5 days to complete and is performed using a dissolved oxygen test kit. The BOD level is determined by comparing the DO level of a water sample taken immediately with the DO level of a water sample that has been incubated in a dark location for 5 days. The difference between the two DO levels represents the amount of oxygen required for the decomposition of any organic material in the sample and is a good approximation of the BOD level.